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Background and aim: The correlation between Covid-19 and nonrespiratory bacteria is mostly unexplored. The following considerations led us to investigate a possible correlation between Hp and SARS-CoV2: both are microbial agents with a very large-scale epidemiology;both can cause GI symptoms;both can persist in the GI tract for long time [Fei Xiao, 2022];patients with pre-existing HP gastric colonization, once infected by SARS-CoV-2, complain more frequently and with greater severity of GI symptoms such as abdominal pain and diarrhea [Balamtekin, 2019];SARS-CoV-2 binds ACE-2 receptors to enter cells, which are widely expressed in the GI tract. In addition, Hp is known to increase the expression of ACE-2 receptors. This study aims to investigate, by C13 Urea BT, the prevalence of Hp infection and the DOB (delta over baseline), in pre-pandemic period (pPP), from Sept. 2017 to Dec. 2019, and during Covid-19 pandemic period (PP), from Jan. 2020 to Apr. 2022, to evaluate whether SARS-CoV-2 and Hp infection association is only due to chance or whether represents a pathogenetic correlation. Material(s) and Method(s): This is a retrospective preliminary study on 1532 randomized patients: 825 and 707 referring respectively to pPP and PP. Result(s): 316 patients underwent C13 Urea BT for the diagnosis of Hp infection: 36 out of 179 (20.11%), and 74 out of 137 (54.01%), respectively in pPP and in PP, tested positive for Hp. The DOB of patients tested during the PP was 40.4+/-17.5, significantly higher when compared to the mean value found in pPP: 17.4+/-16.5 (p=0.0001). [Figure presented] Conclusion(s): Neglecting the search for Hp, also due to difficulties encountered in this period to access BT, represents a risk condition for gastric diseases, especially considering the remarkable elevation of the prevalence and the DOB caused, somehow, by the SARS-CoV-2 virus. Particularly, the higher the DOB, the higher the bacterial load, but, more significantly, the greater the ability of the bacterial strains to produce urease: the strains with the greatest urease-activity are cagA+ strains (those capable of producing the oncogenic protein involved in the process of carcinogenesis) [Moreno-Ochoa, 2020]. Thus, it can be assumed that the higher the DOB, the greater the risk of developing serious gastric problems in the absence of treatment. So, in conclusion, Sars-Cov-2 and HP infection may influence each other. GI morphological and functional alterations due to Sars-Cov-2 infection, which can promote HP colonization and replication, need further investigation.Copyright © 2023. Editrice Gastroenterologica Italiana S.r.l.
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Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide. There is no effective medication for COVID-19 as of now, so it would be good to take preventive measures that not only boost our immunity but also fight against infections. The use of traditional Chinese medicine in China to treat COVID-19 patients sets the prototype demonstrating that traditional medicines can contribute to prevention and treatment successfully. In India, the Ministry of AYUSH (Ayurveda, Yoga, Unani, Siddha, Homeop-athy) released a self-care advisory during the COVID-19 crisis as a preventive aspect. This review article discusses the therapeutic potential and clinical relevance of some herbs [(Tulsi (Ocimum sanctum), Haridra (Curcuma longa), Tvaka (Cinnamon), Maricha (Piper longum), Shunthi (Zingi-ber officinale), Munakka (Dried grapes), Lavang (Syzigiumaromaticum), Pudina (Mentha arvensis), and Ajwain (Trachyspermum ammi)] advised by AUYSH to take during COVID-19 infection. They are effective in COVID-19 management, therefore, authors have discussed their detailed traditional uses as therapeutics and spotted scientific insight and clinical significance of the herbs mentioned above along with their mechanistic viewpoint, adequately, on a single platform. Provided information could be a treasure to open up a new research arena on natural products to manage human health crises effectively, caused not only by COVID-19 but also by other infectious diseases.Copyright © 2023 Bentham Science Publishers.
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Purpose/Objectives: Gram-negative bacteria including E. coli and P. aeruginosa can survive for months on dry hard surfaces, and SARS viruses can persist for days. These contaminated surfaces along with patients' damaged skin barriers, due to wounds or central line insertion sites, increase the risk healthcare-acquired infections (HAI) and subsequent serious complications. Furthermore, with increased frequency and duration of hospitalizations due to the current pandemic, the number of HAIs is on the rise. Currently there are no antimicrobial surfaces that provide both instant and long-lasting antimicrobial protection against a broad spectrum of infectious microbes. Liquid- or radiation-based disinfection techniques are kill microbes quickly, but their effect does not last long before needing reapplication. Antimicrobial surfaces based on heavy metals remain antimicrobial for long durations, but complete disinfection can take hours. In this work, we developed a new class of plant-inspired antimicrobial surfaces and wound dressings that incorporate plant secondary metabolites capable of rapid disinfection (> 4-log reduction) of common bacteria and viruses and maintain their efficacy over time (> 6 months). Methodology: We developed a method for stabilizing naturally antimicrobial essential oils components from plants such as, alpha terpineol (AT) and cinnamaldehyde (CMA), within a polyurethane polymer. Using a modified standard method for evaluating the performance of different nonporous solids (ISO 22196) and median tissue culture infection dose assay, these antimicrobial polyurethane coatings were tested and found to be effective in killing E. coli, P. aeruginosa, methicillin-resistant S. aureus (MRSA), and SARS-CoV-2. The durability of the coatings was tested by linear abrasion, UV and airflow exposure. Application methods such as spray coating and dip coating allow the coating to be applied to a variety of surfaces. Results: Polyurethane surfaces containing 35% AT content (PU-35%AT) showed a ∼5.8-log reduction in E. coli colony forming units per cm2 (CFU/cm2) in under 2 minutes, a shorter time than common commercial disinfectants. Additionally, when subjected to 8 consecutive rounds of inoculation the PU- 35%AT surface reduced the E. coli by >99.99% for all 8 rounds. We achieved a ∼5.8-log reduction of MRSA within 5 minutes on PU-60%AT. The PU-35%AT surfaces showed a 4.0-log reduction in SARS-CoV- 2 in 60 minutes. A PU-70%AT showed a 1.6-log reduction after 10 minutes and maintained virucidal capabilities after 2 weeks. PU+35%AT surfaces maintained a ∼5.3-log reduction in CFU/cm2 in MRSA and E. coli after 1000 abrasion cycles, 12 hours of UV exposure, 25 hours of exposure to -17°C, or 5 months of air flow. Lastly, to demonstrate the coating's real world functionality the PU+35%AT coating was successfully applied to a computer keyboard, cell phone screen protector and medical gauze. Conclusion/Significance: This work demonstrates a novel approach for fabricating a broad-spectrum antibacterial and antiviral polymer surface based on plant essential oil components. This antimicrobial polyurethane coating has not only rapid bactericidal and virucidal capabilities but maintains this efficacy over time. Additionally, the coating can be applied to a variety of surfaces including medical gauze to create wound dressings that significantly reduce bacterial burden and decrease chances of HAIs.
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introduction. The hospital surfaces can contribute to secondary cross contamination, through the hands of health care personnel or contaminated material. It has been necessary to develop ambiental automatic decontamination equipment without the problems related with manual disinfection. material and method. An analytical, prospective, cross-sectional study was carried out, to evaluate the the efficacy and effectiveness of ambiental decontamination at the hospital, with ultraviolet radiation of a 245 nm wavelength equipment, on two semi critical areas, meant for clinical attention to patients with sars-cov-2 infection, with pre and post intervention sampling. 2-stage study to evaluate efficiency and effectiveness. results. In stage 1 (efficacy evaluation) atcc reference strains were sown, subsequently intervention with uv radiation was carried out, the growth of microorganisms was 0/3 in the micas, that is;it was possible to eliminate the bacterial load in 100% (3 samples). In stage 2 (evaluation of effectiveness), microbiological samples were taken from 15 clinic sites under baseline conditions, it was found that there was growth of microorganisms on 12/15 surfaces prior to intervention. After decontamination with uv radiation, the growth of microorganisms was 4/15 in the micas, bacterial elimination was achieved in 73.34% of the sampled surfaces and the bacterial load persisted in 26.66% of the sampled areas. After the decontamination intervention through uv light, a significant bacterial count elimination was achieved (Chi Square test p = 0.0002). conclusions. The decontamination process with uv light in closed spaces with equipment that emits radiation with a wavelength of 254 nm for 30 minutes was an effective intervention to reduce and eliminate the bacterial load from hospital surfaces.
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Introduction Obtaining samples of Neisseria gonorrhoeae for antibiotic sensitivity testing is important for purposes of antimicrobial stewardship. While urethral and cervical gonorrhoea culture samples are usually taken by a healthcare professional, the Covid-19 pandemic necessitated a reduction in direct patient contact. In our service, patients with confirmed gonorrhoea who did not otherwise require examination were asked to take their own urethral or vaginal culture swabs. Methods GUMCAD coding was used to identify cases of cervical or male urethral Neisseria gonorrhoeae infection diagnosed on nucleic acid amplification testing, where the gonococcal culture result and the identity of the swab-taker (patient/healthcare professional) were recorded in the notes. 50 cases were selected in 2019, and 50 in 2020, after the onset of the Covid-19 pandemic. Proportions of patients taking their own swabs were calculated for the two periods. Culture positivity rates were compared between self-taken and healthcare professional-taken swabs. Results During the pandemic, use of self-taken culture samples increased ten-fold. Although positivity for Neisseria gonorrhoeae was lower for self-taken swabs, 38% of male self-taken urethral samples tested positive for gonorrhoea, and the organism was successfully cultured from a self-taken vaginal swab on one occasion. Discussion Although self-taken gonorrhoea cultures were less likely to grow Neisseria gonorrhoeae than those taken by healthcare professionals, they proved a useful tool in gathering sensitivity data in a time of restricted patient contact. The lower positivity rate of self-taken cultures may be partially attributable to their use in patients with fewer symptoms, and hence a lower bacterial load. (Table Presented).
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Background: As of early August 2021, more than 190 million people have developed coronavirus disease (COVID-19), a pandemic that has killed approximately 4 million people. Caused by acute respiratory syndrome coronavirus 2 (SARS-CoV-2), COVID-19 exhibited a highly variable clinical course, ranging from a high proportion of asymptomatic and mild infections to severe and fatal disease. However, the immunological determinants underlying the heterogeneity of COVID-19 remain to be fully elucidated. Methods: To systemically analyze the immunopathogenesis of COVID-19, a multicompartment mathematical model based on both immunological principles and COVID-19-related work performed by the scientific community was built to illustrate the dynamics of host immunity after SARS-CoV-2 infection. We used ordinary differential equations (ODEs) to simulate the time-dependent functions of immunologic variations in the four compartments, which were draining lymph nodes, peripheral blood, lung and distant lymph nodes and spleen. Our model consisted of equations for 109 immunologic variations, which contained 223 parameters. K was used to characterize the adequacy of the SARS-CoV-2-specific naïve T/B cell pool;K I represented the hill coefficient of antigen-presenting cell (APC) differentiation. Further, we used method of pseudo landscape to visualize the effect of APC capacity and the SARS-CoV-2-specific naïve T/B cell pool on clinical outcomes. Results: Based on both immunologic knowledge and extensive COVID-19-related work performed by the scientific community, we constructed a knowledge-driven mathematical model that incorporated SARS-CoV-2 infection, bacterial infection, leukocyte chemotaxis, innate immunity and adaptive immunity. The model simulated and predicted the different trajectories of the viral load, bacterial load, immune cells, cytokines and infected epithelial cells in patients with different severities. A higher viral load and longer virus-shedding period were observed in patients with higher severity, along with an increase in SARS-CoV-2-infected lung epithelial cells. The trajectories of both peripheral blood IL-6 and lymphocytes predicted COVID-19 outcomes. Based on the distribution, trafficking and differentiation of immune cells after SARS-CoV-2 infection, we proposed that early-stage lymphopenia is related to lymphocyte chemotaxis. The delayed initiation of both innate and adaptive immunity resulted in elevated SARS-CoV-2 shedding and was a pivotal cause of COVID-19 severity. Spatiotemporally, viral shedding and postviral bacterial infection evoked stronger innate immunity. Viral shedding could be restrained by the rapid initiation of APC, antibody-secreting cell (ASC) and cytotoxic T cell (CTL). Moreover, our model predicted that the insufficient SARS-CoV-2-specific naïve T/B cell pools and inactive APC caused a series of chain reactions, including viral shedding, bacterial infection, sepsis and cytokine storms. Finally, pseudopotential analysis revealed that a high state characterized by severe bacterial infections and cytokine storms was a stable attractor for patients with insufficient SARS-CoV-2-specific naïve T/B cells and inactive APC (Figure 1). Conclusion: Overall, our analysis provided a comprehensive view of the dynamics of host immunity after SARS-CoV-2 infection and highlighted that the antigen-specific naïve T/B cell pool and APC ability may essentially determine COVID-19 heterogeneity from an immunological standpoint. [Formula presented] Disclosures: No relevant conflicts of interest to declare.
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During the current COVID-19 pandemic, the use of face masks has become increasingly recommended and even mandatory in community settings. To evaluate the risk of bacterial cross-contamination, this study analyzed the bacterial bioburden of disposable surgical masks and homemade cotton masks, and surveyed the habits and face mask preferences of the Flemish population. Using culture approaches and 16S rRNA gene amplicon sequencing, we analyzed the microbial community on surgical and/or cotton face masks of 13 healthy volunteers after 4 h of wearing. Cotton and surgical masks contained on average 1.46 × 105 CFU/mask and 1.32 × 104 CFU/mask, respectively. Bacillus, Staphylococcus, and Acinetobacter spp. were mostly cultured from the masks and 43% of these isolates were resistant to ampicillin or erythromycin. Microbial profiling demonstrated a consistent difference between mask types. Cotton masks mainly contained Roseomonas, Paracoccus, and Enhydrobacter taxa and surgical masks Streptococcus and Staphylococcus. After 4 h of mask wearing, the microbiome of the anterior nares and the cheek showed a trend toward an altered beta-diversity. According to dedicated questions in the large-scale Corona survey of the University of Antwerp with almost 25,000 participants, only 21% of responders reported to clean their cotton face mask daily. Laboratory results indicated that the best mask cleaning methods were boiling at 100°C, washing at 60°C with detergent or ironing with a steam iron. Taken together, this study suggests that a considerable number of bacteria, including pathobionts and antibiotic resistant bacteria, accumulate on surgical and even more on cotton face masks after use. Based on our results, face masks should be properly disposed of or sterilized after intensive use. Clear guidelines for the general population are crucial to reduce the bacteria-related biosafety risk of face masks, and measures such as physical distancing and increased ventilation should not be neglected when promoting face mask use.
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An evident underestimation of the targeted prevention of dental diseases is strongly supported by alarming epidemiologic statistics globally. For example, epidemiologists demonstrated 100% prevalence of dental caries in the Russian population followed by clinical manifestation of periodontal diseases. Inadequately provided oral health services in populations are caused by multi-factorial deficits including but not limited to low socio-economic status of affected individuals, lack of insurance in sub-populations, insufficient density of dedicated medical units. Another important aspect is the "participatory" medicine based on the active participation of population in maintaining oral health: healthcare will remain insufficient as long as the patient is not motivated and does not feel responsible for their oral health. To this end, nearly half of chronically diseased people do not comply with adequate medical services suffering from severely progressing pathologies. Noteworthy, the prominent risk factors and comorbidities linked to the severe disease course and poor outcomes in COVID-19-infected individuals, such as elderly, diabetes mellitus, hypertension and cardiovascular disease, are frequently associated with significantly altered oral microbiome profiles, systemic inflammatory processes and poor oral health. Suggested pathomechanisms consider potential preferences in the interaction between the viral particles and the host microbiota including oral cavity, the respiratory and gastrointestinal tracts. Since an aspiration of periodontopathic bacteria induces the expression of angiotensin-converting enzyme 2, the receptor for SARS-CoV-2, and production of inflammatory cytokines in the lower respiratory tract, poor oral hygiene and periodontal disease have been proposed as leading to COVID-19 aggravation. Consequently, the issue-dedicated expert recommendations are focused on the optimal oral hygiene as being crucial for improved individual outcomes and reduced morbidity under the COVID-19 pandemic condition. Current study demonstrated that age, gender, socio-economic status, quality of environment and life-style, oral hygiene quality, regularity of dental services requested, level of motivation and responsibility for own health status and corresponding behavioural patterns are the key parameters for the patient stratification considering person-tailored approach in a complex dental care in the population. Consequently, innovative screening programmes and adapted treatment schemes are crucial for the complex person-tailored dental care to improve individual outcomes and healthcare provided to the population.